PLK2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31246
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31246-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PLK2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of PLK2 antibody and Lane 1: A431; 2: 293T; 3: COLO320 cell lysate
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IHC-P: PLK2 antibody testing of human lung cancer tissue
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IHC-P: PLK2 antibody testing of rat intestine tissue
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IHC-P testing of PLK2 antibody and rat brain tissue
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Flow cytometry testing of human A549 cells with PLK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PLK2 antibody.
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Western blot testing of PLK2 antibody and Lane 1: A431; 2: 293T; 3: COLO320 cell lysate
IHC-P: PLK2 antibody testing of human lung cancer tissue
IHC-P: PLK2 antibody testing of rat intestine tissue
IHC-P testing of PLK2 antibody and rat brain tissue
Flow cytometry testing of human A549 cells with PLK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=PLK2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PLK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Polo-like kinase 2, also known as SNK, is an enzyme that in humans is encoded by the PLK2 gene. It is a member of the polo family of serine/threonine protein kinases that have a role in normal cell division. The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 5. Polo-like kinase 2 is involved in synaptic plasticity, centriole duplication and G1/S phase transition. This gene plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signalin: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Polo-like kinase 2 (SSELKNRMEYALNMLLQRCN) was used as the immunogen for this PLK2 antibody.
Limitation:
This PLK2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9NYY3