PNP Antibody / Purine Nucleoside Phosphorylase

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NSJ Bioreagents
Product Code: NSJ-R32597
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32597-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Purine nucleoside phosphorylase antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human renal cancer tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
2 / 7
IHC testing of FFPE human liver cancer tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 7
Western blot testing of 1) human 293T, 2) human PC-3 and 3) rat liver lysate using Purine nucleoside phosphorylase antibody at 0.5ug/ml. Predicted molecular weight: 32-34 kDa.
4 / 7
IHC testing of FFPE mouse kidney tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 7
IHC testing of FFPE rat kidney tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
6 / 7
Flow cytometry testing of human U-251 MG cells with Purine Nucleoside Phosphorylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Purine Nucleoside Phosphorylase antibody.
7 / 7
Flow cytometry testing of human U937 MG cells with Purine Nucleoside Phosphorylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Purine Nucleoside Phosphorylase antibody.
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IHC testing of FFPE human renal cancer tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human liver cancer tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human 293T, 2) human PC-3 and 3) rat liver lysate using Purine nucleoside phosphorylase antibody at 0.5ug/ml. Predicted molecular weight: 32-34 kDa.
IHC testing of FFPE mouse kidney tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE rat kidney tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-251 MG cells with Purine Nucleoside Phosphorylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Purine Nucleoside Phosphorylase antibody.
Flow cytometry testing of human U937 MG cells with Purine Nucleoside Phosphorylase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=Purine Nucleoside Phosphorylase antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Purine nucleoside phosphorylase antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
The PNP gene encodes Purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T?lymphocyte function and neurologic dysfunction.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 161-189 (AMSDAYDRTMRQRALSTWKQMGEQRELQE-human) were used as the immunogen for the Purine nucleoside phosphorylase antibody.
Limitation:
This antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P00491