PP2A Antibody

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NSJ Bioreagents
Product Code: NSJ-R31760
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31760-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PP2A antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with PP2A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC staining of FFPE human thyroid cancer tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human esophageal squamous carcinoma tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human colorectal adenocarcinoma tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE mouse brain tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE rat brain tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human 293T, 4) human HeLa, 5) human HepG2, 6) rat brain, 7) rat PC-12, 8) mouse lung, 9) mouse liver, 10) mouse brain and 11) mouse NIH 3T3 cell lysate with PP2A antibody. Predicted molecular weight ~35 kDa.
8 / 8
Flow cytometry testing of fixed and permeabilized human K562 cells with PP2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PP2A antibody.
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Immunofluorescent staining of FFPE human U-2 OS cells with PP2A antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human thyroid cancer tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human esophageal squamous carcinoma tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with PP2A antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human Jurkat, 2) human MCF7, 3) human 293T, 4) human HeLa, 5) human HepG2, 6) rat brain, 7) rat PC-12, 8) mouse lung, 9) mouse liver, 10) mouse brain and 11) mouse NIH 3T3 cell lysate with PP2A antibody. Predicted molecular weight ~35 kDa.
Flow cytometry testing of fixed and permeabilized human K562 cells with PP2A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PP2A antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PP2A antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
5515
Immunogen:
Human recombinant PPP2CA protein (AA 1-309) was used as the immunogen for this PP2A antibody.
Limitation:
This PP2A antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat