PRDX3 Antibody (Peroxiredoxin 3)

Contact us
NSJ Bioreagents
Product Code: NSJ-R31762
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31762-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PRDX3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of PRDX3 antibody and Lane 1: rat brain; 2: mouse brain; 3: rat skeletal muscle; 4: mouse skeletal muscle; 5: human U20S; 6: HeLa; 7: SMMC-7721; 8: RH35; 9: A549 lysate. Expected size ~28KD
2 / 7
IHC-P: PRDX3 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 7
ICC testing of FFPE human HeLa cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 7
ICC testing of FFPE human MCF7 cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 7
ICC testing of FFPE human SMMC-7721 cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 7
Immunofluorescent staining of FFPE human U-2 OS cells with PRDX3 antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
7 / 7
Flow cytometry testing of human U937 cells with PRDX3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRDX3 antibody.
❮❯

Western blot testing of PRDX3 antibody and Lane 1: rat brain; 2: mouse brain; 3: rat skeletal muscle; 4: mouse skeletal muscle; 5: human U20S; 6: HeLa; 7: SMMC-7721; 8: RH35; 9: A549 lysate. Expected size ~28KD
IHC-P: PRDX3 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of FFPE human HeLa cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC testing of FFPE human MCF7 cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
ICC testing of FFPE human SMMC-7721 cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Immunofluorescent staining of FFPE human U-2 OS cells with PRDX3 antibody (red) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U937 cells with PRDX3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRDX3 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PRDX3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Peroxiredoxin 3, also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human protein shares 86% amino acid sequence similarity with mouse, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of Peroxiredoxin 3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that Peroxiredoxin 3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that PRDX3 is a MYC target gene that is required to maintain normal mitochondrial function.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
10935
Immunogen:
Human partial recombinant protein (AA 110-256) was used as the immunogen for this PRDX3 antibody.
Limitation:
This PRDX3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat