Profilin 2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31282
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31282-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Profilin 2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Profilin 2 antibody and rat brain lysate. Expected/observed size ~15KD
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IHC-P: Profilin 2 antibody testing of human tonsil tissue
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IHC-P: Profilin 2 antibody testing of human intestinal cancer tissue
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Western blot testing of Profilin 2 antibody and 1) human placenta, 2) rat brain and 3) mouse brain lysate. Expected/observed size ~15 kDa.
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Flow cytometry testing of human A431 cells with Profilin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Profilin 2 antibody.
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Western blot testing of Profilin 2 antibody and rat brain lysate. Expected/observed size ~15KD
IHC-P: Profilin 2 antibody testing of human tonsil tissue
IHC-P: Profilin 2 antibody testing of human intestinal cancer tissue
Western blot testing of Profilin 2 antibody and 1) human placenta, 2) rat brain and 3) mouse brain lysate. Expected/observed size ~15 kDa.
Flow cytometry testing of human A431 cells with Profilin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Profilin 2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Profilin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Profilin-2 is a protein that in humans is encoded by the PFN2 gene. The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. This gene is mapped to 3q25.1. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. Profilin-2 binds to actin and affects the structure of the cytoskeleton. At high concentrations, it prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human PFN2 (NKKAYSMAKYLRDSGF) was used as the immunogen for this Profilin 2 antibody (100% homologous in human, mouse and rat).
Limitation:
This Profilin 2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35080