Prohibitin Antibody

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NSJ Bioreagents
Product Code: NSJ-R31032
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31032-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Prohibitin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Prohibitin antibody and rat samples 1: lung; 2: skeletal muscle; 3: brain; 4: kidney; and 5: human HeLa; 6: (h) MCF-7; 7: (r) PC-12; 8: (h) A549; 9: (h) SMMC-7721 cell lysate. Expected/observed size ~30KD
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IHC-P: Prohibitin antibody testing of human lung cancer tissue
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ICC testing of Prohibitin antibody and HeLa cells
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Western blot testing of Prohibitin antibody and rat samples 1: lung; 2: skeletal muscle; 3: brain; 4: kidney; and 5: human HeLa; 6: (h) MCF-7; 7: (r) PC-12; 8: (h) A549; 9: (h) SMMC-7721 cell lysate. Expected/observed size ~30KD
IHC-P: Prohibitin antibody testing of human lung cancer tissue
ICC testing of Prohibitin antibody and HeLa cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Prohibitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Prohibitin, also known as PHB1, is a protein that in humans is encoded by the PHB gene. White et al.(1991) mapped the gene to chromosome 17 by analysis of human-mouse somatic cell hybrid cell lines using a genomic fragment of human prohibitin DNA isolated from a library using the rat prohibitin cDNA clone. By in situ hybridization, they localized the gene to 17q21. Sato et al.(1992) isolated the human homolog of the rat gene and mapped it to 17q12-q21 by in situ hybridization. Proliferation of tumor cells depends on new blood vessel formation (angiogenesis) that accompanies malignant progression. Anti-cancer therapies using angiogenesis inhibitors or cytotoxic agents targeted to the vasculature of tumors have been evaluated in clinical trials. Although white fat is a nonmalignant tissue, it has the capability to quickly proliferate and expand. Furthermore, it is highly vascularized. Rupnick et al.(2002) showed that nonspecific angiogenesis inhibitors can prevent the development of obesity of mice.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human PHB1 (KLEAAEDIAYQLSRSRN) was used as the immunogen for this Prohibitin antibody (100% homologous in human, mouse and rat).
Limitation:
This Prohibitin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35232