Prolactin Receptor Antibody

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NSJ Bioreagents
Product Code: NSJ-R31199
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31199-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Prolactin Receptor antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Prolactin Receptor antibody and Lane 1: HeLa; 2: MCF-7 cell lysate. Predicted/observed molecular weight: ~68kDa.
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IHC-P: Prolactin Receptor antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 3
IHC-P: Prolactin Receptor antibody testing of rat testis tissue. HIER: steamed with pH6 citrate buffer.
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Western blot testing of Prolactin Receptor antibody and Lane 1: HeLa; 2: MCF-7 cell lysate. Predicted/observed molecular weight: ~68kDa.
IHC-P: Prolactin Receptor antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: Prolactin Receptor antibody testing of rat testis tissue. HIER: steamed with pH6 citrate buffer.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Prolactin Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
PRLR (Prolactin Receptor) is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al.(1989, 1990) demonstrated that the gene resides in the same chromosomal region as the growth hormone receptor (GHR) gene, which has been mapped to 5p13-p12. Cunningham et al.(1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to GHR or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues(histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR (conserved in all PRLRs but not GHRs) are likely zinc-ion ligands.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of mouse Prolactin Receptor (QLGRLDYLDPTCFMH) was used as the immunogen for this Prolactin Receptor antibody (100% rat homology).
Limitation:
This Prolactin Receptor antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q08501