Proprotein Convertase 1/3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31196
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31196-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Proprotein Convertase 1/3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Proprotein Convertase 1/3 antibody; Lane 1: rat liver; 2: (r) thymus; and human samples 3: A549; 4: HeLa; 5: COLO320; 6: PANC cell lysate
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IHC staining of FFPE rat intestine tissue with Proprotein Convertase 1/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Immunofluorescent staining of FFPE human colon cancer with Proprotein Convertase 1/3 antibody (green) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of Proprotein Convertase 1/3 antibody; Lane 1: rat liver; 2: (r) thymus; and human samples 3: A549; 4: HeLa; 5: COLO320; 6: PANC cell lysate
IHC staining of FFPE rat intestine tissue with Proprotein Convertase 1/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human colon cancer with Proprotein Convertase 1/3 antibody (green) and DAPI nuclear counterstain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence (FFPE): 1-2ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Proprotein Convertase 1/3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Proprotein Convertase, Subtilisin/Kexin-Type, 1, also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. It is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al.(1991) mapped the gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al.(1996) noted that PCSK1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PCSK1 mutations, Jackson et al.(2003) concluded that human intestinal absorptive function is dependent on the enzymes activity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Proprotein Convertase 1/3 (DVFYNTKPYKHRDDR) was used as the immunogen for this Proprotein Convertase 1/3 antibody (100% homologous in human, mouse and rat).
Limitation:
This Proprotein Convertase 1/3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P29120