PSD-95 Antibody (PSD95)

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NSJ Bioreagents
Product Code: NSJ-R31419
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31419-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the PSD-95 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Immunofluorescent staining of FFPE rat brain tissue with PSD-95 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC staining of FFPE rat brain tissue with PSD-95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE mouse brain tissue with PSD-95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) rat brain, 2) mouse brain and 3) human U-87 MG cell lysate with PSD-95 antibody. Predicted molecular weight ~80 kDa but routinely observed at 90~95 kDa.
5 / 5
Western blot testing of mouse brain tissue lysate with PSD-95 antibody. Predicted molecular weight ~80 kDa but routinely observed at 90~95 kDa.
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Immunofluorescent staining of FFPE rat brain tissue with PSD-95 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE rat brain tissue with PSD-95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse brain tissue with PSD-95 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) mouse brain and 3) human U-87 MG cell lysate with PSD-95 antibody. Predicted molecular weight ~80 kDa but routinely observed at 90~95 kDa.
Western blot testing of mouse brain tissue lysate with PSD-95 antibody. Predicted molecular weight ~80 kDa but routinely observed at 90~95 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the PSD-95 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
DLG4 (Discs large homolog 4), also known as Post-synaptic density protein 95 (PSD-95) or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human PSD-95 (RRVERREWSRLKAKDW) was used as the immunogen for this PSD-95 antibody (100% homologous in human, mouse and rat).
Limitation:
This PSD-95 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P78352