PTP1B Antibody (N-Terminal Region)

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NSJ Bioreagents
Product Code: NSJ-R32849
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32849-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the PTP1B antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) placenta, 2) MCF-7, 3) Caco-2, 4) K562, 5) U-2 OS, 6) PC-3, 7) HL-60 and 8) A549 cell lysate with PTP1B antibody. Predicted molecular weight ~50 kDa.
2 / 10
Western blot testing of 1) rat kidney and 2) mouse kidney lysate with PTP1B antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
3 / 10
Immunofluorescent staining of FFPE human tonsil with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 10
IHC staining of FFPE human tonsil with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
5 / 10
IHC staining of FFPE human placenta with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 10
IHC staining of frozen human placenta with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human breast cancer with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE mouse spleen with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 10
IHC staining of FFPE rat spleen with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
10 / 10
Flow cytometry testing of human U-2 OS cells with PTP1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTP1B antibody.
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Western blot testing of human 1) placenta, 2) MCF-7, 3) Caco-2, 4) K562, 5) U-2 OS, 6) PC-3, 7) HL-60 and 8) A549 cell lysate with PTP1B antibody. Predicted molecular weight ~50 kDa.
Western blot testing of 1) rat kidney and 2) mouse kidney lysate with PTP1B antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
Immunofluorescent staining of FFPE human tonsil with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human placenta with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of frozen human placenta with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE mouse spleen with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen with PTP1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human U-2 OS cells with PTP1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PTP1B antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the PTP1B antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Tyrosine-protein phosphatase non-receptor type 1, also known as protein-tyrosine phosphatase 1B (PTP1B), is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 4-30 (EKEFEQIDKSGSWAAIYQDIRHEASDF) were used as the immunogen for the PTP1B antibody.
Limitation:
This PTP1B antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat, Human
Uniprot #:
P18031