RAB27A Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4237
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4237-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the RAB27A antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of human 1) HeLa, 2) Jurkat, 3) MCF7, 4) HepG2, 5) A549, 6) rat stomach, 7) rat thymus, 8) mouse thymus and 9) mouse NIH3T3 lysate with RAB27A antibody at 0.5ug/ml. Predicted molecular weight: 25-27 kDa.
2 / 7
IHC testing of FFPE human breast cancer tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 7
IHC testing of FFPE human lung cancer tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 7
IHC testing of FFPE mouse spleen tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 7
IHC testing of FFPE rat small intestine tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 7
IHC testing of FFPE rat spleen tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
7 / 7
Flow cytometry testing of human K562 cells with RAB27A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=RAB27A antibody.
❮❯

Western blot testing of human 1) HeLa, 2) Jurkat, 3) MCF7, 4) HepG2, 5) A549, 6) rat stomach, 7) rat thymus, 8) mouse thymus and 9) mouse NIH3T3 lysate with RAB27A antibody at 0.5ug/ml. Predicted molecular weight: 25-27 kDa.
IHC testing of FFPE human breast cancer tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung cancer tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse spleen tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat small intestine tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat spleen tissue with RAB27A antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human K562 cells with RAB27A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=RAB27A antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the RAB27A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ras-related protein Rab-27A is a protein that in humans is encoded by the RAB27A gene. The protein encoded by this gene belongs to the small GTPase superfamily, Rab family. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Mutations in this gene are associated with Griscelli syndrome type 2. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids L98-K216 was used as the immunogen for the RAB27A antibody.
Limitation:
This RAB27A antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P51159