RACK1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31656
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31656-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the RACK1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of RACK1 antibody and Lane 1: mouse liver; 2: rat spleen; 3: (m) spleen; 4: human SMMC-7721; 5: (h) HEPG2; 6: (m) HEPA; 7: (r) RH35 lysate. Predicted/observed size ~35KD
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Western blot testing of RACK1 antibody and recombinant human protein (0.5ng)
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Immunofluorescent staining of FFPE human A431 cells with RACK1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Flow cytometry testing of human A431 cells with RACK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RACK1 antibody.
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Western blot testing of RACK1 antibody and Lane 1: mouse liver; 2: rat spleen; 3: (m) spleen; 4: human SMMC-7721; 5: (h) HEPG2; 6: (m) HEPA; 7: (r) RH35 lysate. Predicted/observed size ~35KD
Western blot testing of RACK1 antibody and recombinant human protein (0.5ng)
Immunofluorescent staining of FFPE human A431 cells with RACK1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with RACK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RACK1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the RACK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Guanine nucleotide-binding protein subunit beta-2-like 1, also known as RACK1, is a 35kDa protein that in humans is encoded by the GNB2L1 gene. It is mapped to 5q35.3. It is a major component of translating ribosomes, which harbor significant amounts of PKC. This gene can provide a physical and functional link between PKC signaling and ribosome activation. GNB2L1/RACK1 is also a mediator of agonist-induced Ca(2+) release. Additionally, it is an essential component of an oxygen-independent mechanism for regulating HIF1A stability. Overexpression of RACK1 and PKC-alpha suppressed CLOCK-BMAL1 transcriptional activity, and it stimulated phosphorylation of BMAL1 by PKC-alpha in vitro.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
10399
Immunogen:
Human partial recombinant protein (AA 2-317) was used as the immunogen for this RACK1 antibody.
Limitation:
This RACK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat