Raf1 Antibody / c-Raf

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NSJ Bioreagents
Product Code: NSJ-R32838
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32838-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the c-Raf antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat kidney, 2) mouse kidney, 3) human MCF7, 4) human 22RV1 and 5) human HeLa lysate with c-Raf antibody at 0.5ug/ml. Predicted molecular weight ~73 kDa.~
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Flow cytometry testing of human HeLa cells with c-Raf antibody at
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Western blot testing of 1) rat kidney, 2) mouse kidney, 3) human MCF7, 4) human 22RV1 and 5) human HeLa lysate with c-Raf antibody at 0.5ug/ml. Predicted molecular weight ~73 kDa.~
Flow cytometry testing of human HeLa cells with c-Raf antibody at

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the c-Raf antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF, c-Raf or Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinasekinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids Y364-Q451 was used as the immunogen for the c-Raf antibody.
Limitation:
This c-Raf antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04049