RASGAP Antibody / RASA1

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NSJ Bioreagents
Product Code: NSJ-R32007
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32007-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the RASGAP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with RASGAP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human U-87 MG cells with RASGAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RASGAP antibody.
3 / 3
Western blot testing of human 1) HeLa, 2) MCF7 and 3) SMMC cell lysate with RASGAP antibody. Expected molecular weight ~116/100 kDa (long/short form).
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Immunofluorescent staining of FFPE human A431 cells with RASGAP antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-87 MG cells with RASGAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RASGAP antibody.
Western blot testing of human 1) HeLa, 2) MCF7 and 3) SMMC cell lysate with RASGAP antibody. Expected molecular weight ~116/100 kDa (long/short form).

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the RASGAP antibody should be determined by the researcher.
Description:
RASA1, also known as RAS p21 protein activator 1 or RASGAP, is a 120-kDa cytosolic human protein. The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids AQQHVLKKLLAITELLQQKQNQYTKTNDVR of human RASA1 were used as the immunogen for the RASGAP antibody.
Limitation:
This RASGAP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P20936