Ribonuclease inhibitor Antibody / RNH1

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NSJ Bioreagents
Product Code: NSJ-R31956
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31956-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the Ribonuclease inhibitor antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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Western blot testing of 1) rat brain, 2) rat testis, 3) rat lung, 4) mouse brain, 5) mouse testis, 6) mouse lung and 7) mouse HEPA1-6 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.
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Western blot testing of 1) human placenta, 2) human HeLa, 3) human SK-O-V3, 4) human Jurkat, 5) human U-87 MG, 6) monkey COS-7, 7) human SW620 and 8) human Caco-2 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.
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Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
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Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
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Flow cytometry testing of human A431 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
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IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Ribonuclease Inhibitor antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Western blot testing of 1) rat brain, 2) rat testis, 3) rat lung, 4) mouse brain, 5) mouse testis, 6) mouse lung and 7) mouse HEPA1-6 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.
Western blot testing of 1) human placenta, 2) human HeLa, 3) human SK-O-V3, 4) human Jurkat, 5) human U-87 MG, 6) monkey COS-7, 7) human SW620 and 8) human Caco-2 cell lysate with Ribonuclease Inhibitor antibody. Expected molecular weight ~50 kDa.
Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
Flow cytometry testing of human A549 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.
Flow cytometry testing of human A431 cells with Ribonuclease Inhibitor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Ribonuclease Inhibitor antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the Ribonuclease inhibitor antibody should be determined by the researcher.
Description:
Ribonuclease inhibitor is an enzyme that in humans is encoded by the RNH1 gene. Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular Rnases. In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin. Ribonuclease inhibitor binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids SLDIQSLDIQCEELSDARWAELLPLLQQCQVVRLDD of human RNH1 were used as the immunogen for the Ribonuclease inhibitor antibody.
Limitation:
This Ribonuclease inhibitor antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P13489