RPSA Antibody / 40S Ribosomal protein SA

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NSJ Bioreagents
Product Code: NSJ-R32562
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32562-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the RPSA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of human 1) HeLa and 2) U-2 OS cell lysate with RPSA antibody at 0.5ug/ml. Routinely observed molecular weight: 37-40 kDa and 67 kDa.
2 / 5
Immunofluorescent staining of FFPE human U-2 OS cells with RPSA antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE human breast cancer with RPSA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 5
Flow cytometry testing of human A431 cells with RPSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPSA antibody.
5 / 5
Flow cytometry testing of human PC-3 cells with RPSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPSA antibody.
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Western blot testing of human 1) HeLa and 2) U-2 OS cell lysate with RPSA antibody at 0.5ug/ml. Routinely observed molecular weight: 37-40 kDa and 67 kDa.
Immunofluorescent staining of FFPE human U-2 OS cells with RPSA antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with RPSA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with RPSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPSA antibody.
Flow cytometry testing of human PC-3 cells with RPSA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RPSA antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
40S ribosomal protein SA is a Ribosomal protein that in humans is encoded by the RPSA gene. This RPSA gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids S2-S138 from the human protein were used as the immunogen for the RPSA antibody.
Limitation:
This RPSA antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P08865