RUNX1T1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4332
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4332-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the RUNX1T1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) human placenta and 2) mouse testis lysate with RUNX1T1 antibody at 0.5ug/ml. Predicted molecular weight ~67 kDa.
2 / 5
IHC testing of FFPE human breast cancer tissue with RUNX1T1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
3 / 5
IHC testing of FFPE mouse brain tissue with RUNX1T1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 5
IF/ICC staining of FFPE human U-2 OS cells with RUNX1T1 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
5 / 5
Flow cytometry testing of human U-2 OS cells with RUNX1T1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RUNX1T1 antibody.
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Western blot testing of 1) human placenta and 2) mouse testis lysate with RUNX1T1 antibody at 0.5ug/ml. Predicted molecular weight ~67 kDa.
IHC testing of FFPE human breast cancer tissue with RUNX1T1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse brain tissue with RUNX1T1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IF/ICC staining of FFPE human U-2 OS cells with RUNX1T1 antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with RUNX1T1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= RUNX1T1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the RUNX1T1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Protein CBFA2T1 is a protein that in humans is encoded by the RUNX1T1 gene. This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids T335-D510 was used as the immunogen for the RUNX1T1 antibody.
Limitation:
This RUNX1T1 antibody is available for research use only.
Localization:
Nucleus
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
Q06455