S100A6 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31897
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31897-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the S100A6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with S100A6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 8
IHC testing of FFPE human breast cancer with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 8
IHC testing of FFPE human placenta with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 8
IHC testing of FFPE mouse testis with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 8
IHC testing of FFPE rat lung with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
6 / 8
Western blot testing of 1) human HeLa, 2) human SW620, 3) human placenta and 4) mouse NIH 3T3 cell lysate with S100A6 antibody. Expected molecular weight ~10 kDa.
7 / 8
Flow cytometry testing of human SiHa cells with S100A6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= S100A6 antibody.
8 / 8
Flow cytometry testing of human A431 cells with S100A6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= S100A6 antibody.
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Immunofluorescent staining of FFPE human A431 cells with S100A6 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human breast cancer with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE human placenta with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE mouse testis with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat lung with S100A6 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of 1) human HeLa, 2) human SW620, 3) human placenta and 4) mouse NIH 3T3 cell lysate with S100A6 antibody. Expected molecular weight ~10 kDa.
Flow cytometry testing of human SiHa cells with S100A6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= S100A6 antibody.
Flow cytometry testing of human A431 cells with S100A6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= S100A6 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the S100A6 antibody should be determined by the researcher.
Description:
S100 calcium-binding protein A6 (S100A6) is a protein that in humans is encoded by the S100A6 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 1-90 of human S100 alpha 6 were used as the immunogen for the S100A6 antibody.
Limitation:
This S100A6 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P06703