SCRIB Antibody / SCRIBBLE

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NSJ Bioreagents
Product Code: NSJ-RQ4322
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4322-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
After reconstitution, the SCRIB antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) MCF7, 2) COLO-320, 3) 22RV1 and 4) SGC-7901 lysate with SCRIB antibody at 0.5ug/ml. Predicted molecular weight ~175 kDa, routinely observed at 200-240 kDa.
2 / 4
Flow cytometry testing of human HepG2 cells with SCRIB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SCRIB antibody.
3 / 4
Flow cytometry testing of human A549 cells with SCRIB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SCRIB antibody.
4 / 4
Immunofluorescent staining of FFPE human U-2 OS cells with SCRIB antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of human 1) MCF7, 2) COLO-320, 3) 22RV1 and 4) SGC-7901 lysate with SCRIB antibody at 0.5ug/ml. Predicted molecular weight ~175 kDa, routinely observed at 200-240 kDa.
Flow cytometry testing of human HepG2 cells with SCRIB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SCRIB antibody.
Flow cytometry testing of human A549 cells with SCRIB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SCRIB antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with SCRIB antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
Optimal dilution of the SCRIB antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
SCRIB, also known as Scribble, SCRIBL, or Scribbled homolog (Drosophila), is a scaffold protein which in humans is encoded by the SCRIB gene. In Drosophila melanogaster, SCRIB is involved in synaptic function, neuroblast differentiation, and epithelial polarization. Mechanistically, the human homolog is a scaffold protein linked to cellular differentiation centered on the regulation of epithelial as well as neuronal morphogenesis. Deficiency in SCRIB impairs many aspects of cell polarity and cell movement. SCRIB is also likely involved in establishing apical-basal polarity as well as progression from the G1 phase to S phase in the cell cycle as a result of its relationship with cell proliferation and exocytosis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids F172-K409 was used as the immunogen for the SCRIB antibody.
Limitation:
This SCRIB antibody is available for research use only.
Localization:
Cell membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q14160