SERT Antibody / Serotonin Transporter / SLC6A4

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NSJ Bioreagents
Product Code: NSJ-R32066
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32066-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SERT antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) human HepG2, 2) human Caco-2, 3) human SW620, 4) human COLO-320, 5) human K562 6) human U-2 OS, 7) human U-87 MG, 8) rat heart, 9) mouse heart and 10) mouse SP2/0 cell lysate with SERT antibody. Expected molecular weight: ~70/85-95 kDa (unmodified/glycosylated).
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IHC testing of FFPE human breast cancer tissue with SERT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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IHC testing of FFPE rat brain with SERT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
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Western blot testing of 1) human HepG2, 2) human Caco-2, 3) human SW620, 4) human COLO-320, 5) human K562 6) human U-2 OS, 7) human U-87 MG, 8) rat heart, 9) mouse heart and 10) mouse SP2/0 cell lysate with SERT antibody. Expected molecular weight: ~70/85-95 kDa (unmodified/glycosylated).
IHC testing of FFPE human breast cancer tissue with SERT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat brain with SERT antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the SERT antibody should be determined by the researcher.
Description:
SLC6A4 (Solute carrier family 6, member 4), also known as SERT (serotonin transporter), is a monoamine transporter protein. The SLC6A4 gene spans 31 kb and contains 14 exons. This protein integral membrane protein transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is the target of many antidepressant medications, including those of the SSRI class. It is a member of the sodium: neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids DDTRHSIPATTTTLVAELHQGERETWGKK of human SLC6A4/SERT were used as the immunogen for the SERT antibody.
Limitation:
This SERT antibody is available for research use only.
Localization:
Cell membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P31645