SIP Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30852
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30852-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SIP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of SIP antibody and Lane 1: rat liver ; 2: (r) brain; 3: (r) spleen; 4: human SMMC-7721; 5: (h) COLO320; 6: (h) SW620; 7: (h) 293T cell lysate
2 / 4
ICC testing of A549 cells with SIP antibody.
3 / 4
IHC testing of FFPE human liver cancer tissue with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.
4 / 4
IHC testing of FFPE rat brain with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.
❮❯

Western blot testing of SIP antibody and Lane 1: rat liver ; 2: (r) brain; 3: (r) spleen; 4: human SMMC-7721; 5: (h) COLO320; 6: (h) SW620; 7: (h) 293T cell lysate
ICC testing of A549 cells with SIP antibody.
IHC testing of FFPE human liver cancer tissue with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.
IHC testing of FFPE rat brain with SIP antibody. HIER: boil sections in pH6 citrate buffer for 20 min and allow to cool before staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SIP antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
CACYBP (Calcyclin-binding protein), also called SIP (SIAH Interacting Protein), is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human SIAH Interacting Protein (NTRWDYLTQVEKECKE) was used as the immunogen for this SIP antibody (100% homologous in human, mouse and rat).
Limitation:
This SIP antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9HB71