SIP1 Antibody (ZEB2)

Contact us
NSJ Bioreagents
Product Code: NSJ-R31062
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31062-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the SIP1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 1
Western blot testing of SIP1 antibody and Lane 1: 293T; 2: HeLa cell lysate. Predicted molecular weight 120~136 kDa.
❮❯

Western blot testing of SIP1 antibody and Lane 1: 293T; 2: HeLa cell lysate. Predicted molecular weight 120~136 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SIP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Zinc finger E-box-binding homeobox 2, also known as Smad Interacting Protein 1 (SIP1) or Zinc finger homeobox 1B (ZFHX1B), is a protein that in humans is encoded by the ZEB2 gene. The gene is a member of the ZEB1/Drosophila Zfh1 family of 2-handed zinc finger/homeodomain proteins and functions as a DNA-binding transcriptional repressor that interacts with activatedSMADs, the transducers ofTGF-betasignaling, and interacts with the nucleosome remodeling and histone deacetylation(NURD) complex. By radiation hybrid analysis, Nagase et al.(1998) mapped the gene to chromosome 2. Wakamatsu et al.(2001) mapped the gene to chromosome 2q22. Vandewalle et al.(2005) showed that expression of mouse Sip1 in human epithelial cells caused a morphologic change from an epithelial to a mesenchymal phenotype. Expression ofSNAI1in epithelial cells triggers an epithelial-mesenchyme transition. Beltran et al.(2008) showed that synthesis of SIP1 was upregulated following SNAI1 expression in human cell lines.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Smad Interacting Protein 1 (DMQQRRKYQRKQGFQ) was used as the immunogen for this SIP1 antibody (100% homologous in human, mouse and rat).
Limitation:
This ZEB2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O60315