SLC9A2 Antibody

NSJ Bioreagents
Product Code: NSJ-R31343
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31343-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SLC9A2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of mouse kidney tissue lysate with SLC9A2 antibody.  Expected molecular weight ~92 kDa.
2 / 4
Western blot testing of rat kidney tissue lysate with SLC9A2 antibody.  Expected molecular weight ~92 kDa.
3 / 4
IHC staining of FFPE human lung cancer tissue with SLC9A2 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE rat kidney tissue with SLC9A2 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Western blot testing of mouse kidney tissue lysate with SLC9A2 antibody.  Expected molecular weight ~92 kDa.
Western blot testing of rat kidney tissue lysate with SLC9A2 antibody.  Expected molecular weight ~92 kDa.
IHC staining of FFPE human lung cancer tissue with SLC9A2 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE rat kidney tissue with SLC9A2 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SLC9A2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sodium?hydrogen exchanger 2, also called SLC9A2 or NHE2 is a protein that in humans is encoded by the SLC9A2 gene. The Na+/H+ exchangers (NHE) are membrane proteins involved in cell volume. The This protein is found in several tissues, including intestine and kidney, and is highly expressed in villus and distal convoluted tubules. It is involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. It seems to play an important role in colonic sodium absorption.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human SLC9A2/NHE2 (RYLSLPKNTKLPEKLQKRR) was used as the immunogen for this SLC9A2 antibody.
Limitation:
This SLC9A2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UBY0