SMAD1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31227
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31227-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SMAD1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of SMAD1 antibody and Lane 1: SMMC-7721; 2: K562; 3: HT1080; 4: HeLa; 5: Jurkat cell lysate. Predicted molecular weight: 52~60 kDa.
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IHC-P: SMAD1 antibody testing of human lung cancer tissue
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ICC testing of SMAD1 antibody and A549 cells
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Western blot testing of SMAD1 antibody and Lane 1: SMMC-7721; 2: K562; 3: HT1080; 4: HeLa; 5: Jurkat cell lysate. Predicted molecular weight: 52~60 kDa.
IHC-P: SMAD1 antibody testing of human lung cancer tissue
ICC testing of SMAD1 antibody and A549 cells

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SMAD1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Mothers against decapentaplegic homolog 1, also known as SMAD family member 1, is a protein that in humans is encoded by the SMAD1 gene. SMAD1 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic(Mad) and the C. elegans gene Sma. The gene was assigned to human chromosome 4q31.21. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of mouse SMAD family member 1 (AEISRGDVQAVAYEE) was used as the immunogen for this Smad1 antibody.
Limitation:
This SMAD1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P70340