SMAD2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30998
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30998-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Smad2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC-P: Smad2 antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
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ICC staining of human HeLa cells with Smad2 antibody.
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Western blot testing of human 1) HeLa, 2) MCF7 and 3) MDA-MB-453 cell lysate with Smad2 antibody. Predicted molecular weight: 52~60 kDa.
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IHC-P: Smad2 antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC staining of human HeLa cells with Smad2 antibody.
Western blot testing of human 1) HeLa, 2) MCF7 and 3) MDA-MB-453 cell lysate with Smad2 antibody. Predicted molecular weight: 52~60 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Smad2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
SMAD family member 2, also known as MADR2, and MADH2 (Mothers against decapentaplegic homolog 2), is a protein that in humans is encoded by the SMAD2 gene. It belongs to the SMAD family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene 'Sma.' The gene has been mapped to 18q21.1, a region which is frequently deleted in colorectal cancers. SMAD2 mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Smad2 (DQWDTTGLYSFSEQTRSLD) was used as the immunogen for this Smad2 antibody (100% homologous in human, mouse and rat).
Limitation:
This Smad2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15796