SMC6 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-RQ4195
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4195-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the SMC6 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 6
Western blot testing of human 1) HeLa, 2) HepG2, 3) PANC-1, 4) SK-OV-3, 5) COLO320, 6) rat testis and 7) mouse testis tissue with SMC6 antibody at 0.5ug/ml. Predicted molecular weight ~126 kDa.
2 / 6
IHC staining of FFPE human testis tissue with SMC6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse testis tissue with SMC6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat testis tissue with SMC6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
5 / 6
Flow cytometry testing of human A431 cells with SMC6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC6 antibody.
6 / 6
Immunofluorescent staining of FFPE human A431 cells with SMC6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
❮❯

Western blot testing of human 1) HeLa, 2) HepG2, 3) PANC-1, 4) SK-OV-3, 5) COLO320, 6) rat testis and 7) mouse testis tissue with SMC6 antibody at 0.5ug/ml. Predicted molecular weight ~126 kDa.
IHC staining of FFPE human testis tissue with SMC6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse testis tissue with SMC6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with SMC6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Flow cytometry testing of human A431 cells with SMC6 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SMC6 antibody.
Immunofluorescent staining of FFPE human A431 cells with SMC6 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml,Immunofluorescence (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the SMC6 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Structural maintenance of chromosomes protein 6, also knowns SMC6L1, is a protein that in humans is encoded by the SMC6 gene. It is involved in the Alternative lengthening of telomeres cancer mechanism. The International Radiation Hybrid Mapping Consortium mapped the SMC6 gene to chromosome 2.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids D205-E443 was used as the immunogen for the SMC6 antibody.
Limitation:
This SMC6 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q96SB8