SNAP23 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30867
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30867-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SNAP23 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of SNAP23 antibody and Lane 1: rat spleen; 2: (r) testis; 3: (r) ovary; 4: human HeLa; 5: (h) MCF-7; 6: (h) SKOV; Expected size: ~23KD
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IHC-P: SNAP23 antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
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ICC: SNAP23 antibody testing K562 cells
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IHC-F: testing of human placenta tissue
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Western blot testing of SNAP23 antibody and Lane 1: rat spleen; 2: (r) testis; 3: (r) ovary; 4: human HeLa; 5: (h) MCF-7; 6: (h) SKOV; Expected size: ~23KD
IHC-P: SNAP23 antibody testing of human placenta tissue. HIER: steamed with pH6 citrate buffer.
ICC: SNAP23 antibody testing K562 cells
IHC-F: testing of human placenta tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SNAP23 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Synaptosomal-Associated Protein, 23-KD is a protein that in humans is encoded by the SNAP23 gene. The gene has 8 exons, with the initiation codon located in exon 2 and is mapped on 15q15.1-q15.2. Its amino acid sequence is 59% identical to that of SNAP25. Northern blot analysis revealed that SNAP23 is ubiquitously expressed. The protein is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins. After relocation, it is required for exocytosis, implying a crucial role in promoting membrane fusion. TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells. STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin. It is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs. It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human SNAP23 (DTNRDRIDIANARAKKLIDS) was used as the immunogen for this SNAP23 antibody.
Limitation:
This SNAP23 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O00161