SOD2 Antibody Superoxide Dismutase 2

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NSJ Bioreagents
Product Code: NSJ-R30869
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30869-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SOD2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of SOD2 antibody and Lane 1: rat liver; 2: (r) intestine; 3: (r) lung; 4: (r) heart; 5: human SMMC-7721; 6: (h) HeLa; 7: (h) COLO320; 8: (h) SW620; 9: (h) A549 cell lysate. Predicted molecular weight ~ 25 kDa.
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IHC-P: SOD2 antibody testing of human intestinal cancer tissue
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IHC-P: SOD2 antibody testing of rat brain tissue
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Western blot testing of SOD2 antibody and Lane 1: rat liver; 2: (r) intestine; 3: (r) lung; 4: (r) heart; 5: human SMMC-7721; 6: (h) HeLa; 7: (h) COLO320; 8: (h) SW620; 9: (h) A549 cell lysate. Predicted molecular weight ~ 25 kDa.
IHC-P: SOD2 antibody testing of human intestinal cancer tissue
IHC-P: SOD2 antibody testing of rat brain tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SOD2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Superoxide Dismutase 2, also called IPO-B or MNSOD, is a mitochondrial matrix enzyme that scavenges oxygen radicals produced by the extensive oxidation-reduction and electron transport reactions occurring in mitochondria. This gene is a member of the iron/manganese superoxide dismutase family. Using a somatic cell hybrid panel containing different segments of chromosome 6, they demonstrated that SOD2 is located in the region 6q25.3-qter which, together with the FISH analysis, indicated that the enzyme is in the distal portion of 6q25. The gene encodes an intramitochondrial free radical scavenging enzyme that is the first line of defense against superoxide produced as a byproduct of oxidative phosphorylation. Adeno-associated viral delivery of the human gene resulted in suppression of optic nerve degeneration and rescue of retinal ganglion cells. The findings suggested that reactive oxygen species contributed to retinal cell death and optic nerve damage in mice with complex I deficiency, and that expression of SOD2 attenuated the disease process.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human SOD2 (QIMQLHHSKHHAAYVNNL) was used as the immunogen for this SOD2 antibody (100% mouse homology).
Limitation:
This SOD2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P04179