SPHK2 Antibody

NSJ Bioreagents
Product Code: NSJ-RQ4299
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4299-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the SPHK2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) COLO-320, 2) HepG2 and 3) 22RV1 cell lysate with SPHK2 antibody at 0.5ug/ml. Predicted molecular weight ~69 kDa.
2 / 2
Flow cytometry testing of human A549 cells with SPHK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SPHK2 antibody.

Western blot testing of human 1) COLO-320, 2) HepG2 and 3) 22RV1 cell lysate with SPHK2 antibody at 0.5ug/ml. Predicted molecular weight ~69 kDa.
Flow cytometry testing of human A549 cells with SPHK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SPHK2 antibody.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the SPHK2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Sphingosine kinase 2 is a protein that in humans is encoded by the SPHK2 gene. This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids F215-M269 was used as the immunogen for the SPHK2 antibody.
Limitation:
This SPHK2 antibody is available for research use only.
Localization:
Cytoplasm, membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
Q9NRA0