Splicing factor 1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30963
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30963-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Splicing factor 1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE rat small intestine tissue with Splicing factor 1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung cancer tissue with Splicing factor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of frozen rat spleen tissue with Splicing factor 1 antibody.
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Immunocytochemical staining of FFPE human HeLa cells with Splicing factor 1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
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Immunofluorescent staining of FFPE human U-87 MG cells with Splicing factor 1 antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
6 / 7
Western blot testing of 1) human K562, 2) human A549, 3) human ThP-1, 4) rat PC-12 and 5) mouse RAW264.7 cell lysate with Splicing factor 1 antibody. Predicted molecular weight ~68 kDa (multiple isoforms).
7 / 7
Flow cytometry testing of human 293T cells with Splicing factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Splicing factor 1 antibody.
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IHC staining of FFPE rat small intestine tissue with Splicing factor 1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with Splicing factor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of frozen rat spleen tissue with Splicing factor 1 antibody.
Immunocytochemical staining of FFPE human HeLa cells with Splicing factor 1 antibody. HIER: boil tissue sections in pH6 citrate buffer for 20 min and allow to cool before testing.
Immunofluorescent staining of FFPE human U-87 MG cells with Splicing factor 1 antibody (red). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human K562, 2) human A549, 3) human ThP-1, 4) rat PC-12 and 5) mouse RAW264.7 cell lysate with Splicing factor 1 antibody. Predicted molecular weight ~68 kDa (multiple isoforms).
Flow cytometry testing of human 293T cells with Splicing factor 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Splicing factor 1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Splicing factor 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Splicing factor 1, also known a sSF1, Zinc finger protein 162 (ZNF162), ZFM162, Transcription factor ZFM1, and Mammalian branch point-binding protein (BBP or mBBP), is a protein that in humans is encoded by the SF1 gene. It ispredicted 623-amino acid protein. RT-PCR was used to show expression in the thyroid gland, pancreas, adrenal gland, and ovary. All of the isoforms contain a KH domain, a sequence motif present in proteins playinig a major role in regulating cellular RNA metabolism.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of human Splicing factor 1 (DFPSKKRKRSRWNQDTMEQK) was used as the immunogen for this Splicing factor 1 antibody (100% homologous in human, mouse and rat).
Limitation:
This Splicing factor 1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15637