Src Antibody

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NSJ Bioreagents
Product Code: NSJ-R30158
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30158-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the Src antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human U-2 OS cells with SRC-1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human SiHa cells with SRC-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRC-1 antibody.
3 / 3
Western blot testing of 1) human SKO-V-3, 2) human SGC-7901, 3) human Jurkat, 4) rat kidney and 5) mouse NIH 3T3 lysate. Predicted molecular weight ~157 kDa.
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Immunofluorescent staining of FFPE human U-2 OS cells with SRC-1 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human SiHa cells with SRC-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SRC-1 antibody.
Western blot testing of 1) human SKO-V-3, 2) human SGC-7901, 3) human Jurkat, 4) rat kidney and 5) mouse NIH 3T3 lysate. Predicted molecular weight ~157 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the SRC-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The nuclear receptor coactivator 1 (NCOA1), also known as Src1, is a transcriptional coregulatory protein that contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. Src is recruited to DNA promotion sites by ligand-activated nuclear receptors. It in turn acylates histones, which makes downsteam DNA more accessible to transcription. Hence, it assists nuclear receptors in the upregulation of DNA expression. It has been found that the protein can enhance the transcriptional activity of ligand-bound PGR but does not alter the basal activity of the target promoter. It also enhances estrogen receptor, glucocorticoid receptor, thyroid hormone receptor, and retinoid X receptor transcriptional activities through their cognate DNA response elements in the presence of hormone. Additionally, Src may play a role as a bridging molecule between nuclear hormone receptors and general transcription factors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
8648
Immunogen:
Human partial recombinant protein (AA 614-826) was used as the immunogen for this Src antibody.
Limitation:
This SRC-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat