Src Antibody

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NSJ Bioreagents
Product Code: NSJ-R31489
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31489-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Src antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
IHC staining of FFPE human rectal cancer tissue with Src antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human liver cancer tissue with Src antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human 1) A549, 2) RT4, 3) PC-3 and 4) SH-SY5Y cell lysate with Src antibody. Predicted molecular weight: 55-60 kDa.
4 / 4
Flow cytometry testing of human HepG2 cells with Src antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Src antibody.
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IHC staining of FFPE human rectal cancer tissue with Src antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Src antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) RT4, 3) PC-3 and 4) SH-SY5Y cell lysate with Src antibody. Predicted molecular weight: 55-60 kDa.
Flow cytometry testing of human HepG2 cells with Src antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Src antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Src antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Sarcoma is a proto-oncogenic tyrosine kinase originally discovered by J. Michael Bishop and Harold E. Varmus. It belongs to a family of non-receptor tyrosine kinases called Src family kinases. The human proto-oncogene was assigned to chromosome 20. Its gene is mapped to 20q12-q13. The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry. This proto-oncogene may play a role in the regulation of embryonic development and cell growth.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
6714
Immunogen:
Human partial recombinant protein (AA 2-368) was used as the immunogen for this Src antibody.
Limitation:
This Src antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human