SSR3 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30894
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30894-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SSR3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human placental tissue with SSR3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 12
Immunofluorescent staining of FFPE mouse brain tissue with SSR3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 12
Immunofluorescent staining of FFPE rat brain tissue with SSR3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 12
IHC staining of FFPE human placental tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 12
IHC staining of FFPE human lung cancer tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 12
IHC staining of FFPE human breast cancer tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 12
IHC staining of FFPE rat hippocampus tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 12
IHC staining of FFPE rat cerebral cortex tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 12
IHC staining of FFPE mouse hippocampus tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
10 / 12
IHC staining of FFPE mouse cerebral cortex tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 12
ICC staining of FFPE human HeLa cells with SSR3 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
12 / 12
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human K562, 4) monkey COS-7, 5) rat C6 and 6) RAW264.7 cell lysate with SSR3 antibody. Expected molecular weight ~20 kDa.
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Immunofluorescent staining of FFPE human placental tissue with SSR3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE mouse brain tissue with SSR3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Immunofluorescent staining of FFPE rat brain tissue with SSR3 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human placental tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung cancer tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat hippocampus tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat cerebral cortex tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse hippocampus tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse cerebral cortex tissue with SSR3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
ICC staining of FFPE human HeLa cells with SSR3 antibody. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human HeLa, 2) human HepG2, 3) human K562, 4) monkey COS-7, 5) rat C6 and 6) RAW264.7 cell lysate with SSR3 antibody. Expected molecular weight ~20 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the SSR3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Signal sequence receptor, gamma is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al.(1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross(2012) mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37). Hartmann et al.(1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 8-23 (KQQSEEDLLLQDFSRN) were used as the immunogen for this SSR3 antibody (100% homologous in human, mouse and rat).
Limitation:
This SSR3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UNL2