STAT5a Antibody

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NSJ Bioreagents
Product Code: NSJ-R30938
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30938-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the STAT5a antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1: HeLa; 2: COLO320; 3: Jurkat; 4: CEM cell lysate with STAT5a antibody. Predicted molecular weight: ~91 kDa.
2 / 3
Flow cytometry testing of human Jurkat cells with STAT5a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT5a antibody.
3 / 3
Flow cytometry testing of human U-2 OS cells with STAT5a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT5a antibody.
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Western blot testing of human 1: HeLa; 2: COLO320; 3: Jurkat; 4: CEM cell lysate with STAT5a antibody. Predicted molecular weight: ~91 kDa.
Flow cytometry testing of human Jurkat cells with STAT5a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT5a antibody.
Flow cytometry testing of human U-2 OS cells with STAT5a antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= STAT5a antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the STAT5a antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
STAT5 (Signal transducer and activator of transcription 5) also known as STAT5a, is a protein that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. Hou et al.(1995) cloned the human cDNA from an umbilical vein endothelial cell library and found that it encodes a 794-amino acid polypeptide with a predicted mass of approximately 90.5 kD. To analyze the possible role of STAT5 in human GH-induced proliferation,Friedrichsen et al.(2001) expressed a dominant-negative STAT5 mutant, STAT5Adelta-749, in INS-1 cells under the control of a doxycycline-inducible promoter by stable transfection. Chromatin immunoprecipitation analysis confirmed direct binding of STAT5 to the HIF2A promoter 344 bp upstream of the HIF2A start site. STAT5 overexpression independently resulted in erythroid commitment in megakaryocytic-erythroid progenitors, which was abrogated by knockdown of GATA1. The protein is responsible for the immediate-early induction of the long isoform of the BCL2-related protein in erythroid cells through direct binding to the promoter of the BCLX gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human STAT5a (YNMYPQNPDHVLDQDGE) was used as the immunogen for this STAT5a antibody.
Limitation:
This STAT5a antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P42229