Surfactant protein D Antibody

NSJ Bioreagents
Product Code: NSJ-R32075
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32075-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Surfactant protein D antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
Western blot testing of 1) rat lung, 2) rat brain and 3) human PANC lysate with Surfactant protein D antibody. Predicted/observed molecular weight ~38 kDa.
2 / 5
IHC testing of FFPE human lung cancer tissue with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 5
IHC testing of frozen human placental tissue with Surfactant protein D antibody.
4 / 5
ICC testing of FFPE human HeLa cells with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
5 / 5
ICC testing of FFPE human A549 cells with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

Western blot testing of 1) rat lung, 2) rat brain and 3) human PANC lysate with Surfactant protein D antibody. Predicted/observed molecular weight ~38 kDa.
IHC testing of FFPE human lung cancer tissue with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placental tissue with Surfactant protein D antibody.
ICC testing of FFPE human HeLa cells with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
ICC testing of FFPE human A549 cells with Surfactant protein D antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,ELISA: 0.1-0.5ug/ml, Immunohistochemistry (Frozen): 0.5-1ug/ml,ICC (Paraffin): 0.5-1ug/ml
Application Note:
Optimal dilution of the Surfactant protein D antibody should be determined by the researcher.
Description:
Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. It is mapped to 10q22.2-q23.1. The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RSAAENAALQQLVVAKNEAAFLSMTD SKTE of human Surfactant protein D were used as the immunogen for the Surfactant protein D antibody.
Limitation:
This Surfactant protein D antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Rat
Uniprot #:
P35247