Survivin Antibody

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NSJ Bioreagents
Product Code: NSJ-R32641
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32641-100ug100ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the Survivin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat PC-12, 2) human K562 and 3) mouse thymus cell lysate with Survivin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
2 / 4
Western blot testing of human 293T cell lysate with Survivin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
3 / 4
IHC staining of FFPE human intestinal cancer with Survivin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
4 / 4
IHC staining of FFPE human intestinal cancer with Survivin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of 1) rat PC-12, 2) human K562 and 3) mouse thymus cell lysate with Survivin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
Western blot testing of human 293T cell lysate with Survivin antibody at 0.5ug/ml. Predicted molecular weight ~16 kDa.
IHC staining of FFPE human intestinal cancer with Survivin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human intestinal cancer with Survivin antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA: 0.1-0.5ug/ml (human protein tested; request BSA-free format for coating)
Application Note:
Optimal dilution of the Survivin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is a protein that in humans encoded by the BIRC5 gene. It is a member of the inhibitor of apoptosis (IAP) family. The survivin gene contains 4 exons. This gene is mapped to chromosome 17q25 by pulsed field gel electrophoresis and single- and 2-color FISH. The survivin protein functions as inhibitor caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. And this protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids M1-D142 from the human protein were used as the immunogen for the Survivin antibody.
Limitation:
This Survivin antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
O15392