Synaptophysin Antibody

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NSJ Bioreagents
Product Code: NSJ-R30080
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30080-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: SVP-38, aka Syn-58
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Synaptophysin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Synaptophysin antibody and rat brain tissue lysate
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IHC testing of FFPE human glioma tissue with Synaptophysin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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IHC testing of FFPE human meningeoma tissue with Synaptophysin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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IHC testing of FFPE rat brain with Synaptophysin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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Western blot testing of Synaptophysin antibody and rat brain tissue lysate
IHC testing of FFPE human glioma tissue with Synaptophysin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE human meningeoma tissue with Synaptophysin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat brain with Synaptophysin antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,IHC (Paraffin): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml, formalin or acetone fixed tissues
Application Note:
The stated application concentrations are suggested starting points. Titration of the Synaptophysin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Synaptophysin (SYP) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. Ozcelik et al.(1990) concluded that the gene has 7 exons distributed over about 20 kb. The SYP locus mapped to Xp11.23-p11.22. Regionalization of the gene on the X chromosome was also done with hamster/human hybrid cells in which various portions of the human X chromosome were present.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
6855
Immunogen:
Rat retina synaptosome was used as the immunogen for this Synaptophysin antibody.
Limitation:
This Synaptophysin antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, rat