Synaptotagmin 1 Antibody / SYT1

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NSJ Bioreagents
Product Code: NSJ-RQ4208
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4208-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the SYT1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat brain and 2) mouse brain lysate with SYT1 antibody at 0.5ug/ml. Expected molecular weight: 48-65 kDa depending on glycosylation level.
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IHC testing of FFPE human glioma with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE mouse brain tissue with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 8
IHC testing of FFPE rat brain tissue with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 8
IHC testing of FFPE human melanoma with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 8
Flow cytometry testing of human A549 cells with SYT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SYT1 antibody.
7 / 8
Flow cytometry testing of human PC-3 cells with SYT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SYT1 antibody.
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Flow cytometry testing of mouse LLC1 cells with SYT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SYT1 antibody.
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Western blot testing of 1) rat brain and 2) mouse brain lysate with SYT1 antibody at 0.5ug/ml. Expected molecular weight: 48-65 kDa depending on glycosylation level.
IHC testing of FFPE human glioma with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse brain tissue with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat brain tissue with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human melanoma with SYT1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human A549 cells with SYT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SYT1 antibody.
Flow cytometry testing of human PC-3 cells with SYT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SYT1 antibody.
Flow cytometry testing of mouse LLC1 cells with SYT1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=SYT1 antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the SYT1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RRPIAQWHTLQVEEEVDAMLAVKK were used as the immunogen for the SYT1 antibody.
Limitation:
This SYT1 antibody is available for research use only.
Localization:
Cytoplasm, secretory vesicle membrane
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P21579