Syndecan 3 Antibody

NSJ Bioreagents
Product Code: NSJ-R31248
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31248-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Syndecan 3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of 1) human U-2 OS, 2) rat PC-12 and 3) mouse NIH 3T3 cell lysate with Syndecan 3 antibody. Predicted molecular weight: 46 kDa, observed molecular weight: 60-70 kDa.
2 / 3
IHC staining of FFPE human placental tissue with Syndecan 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 3
IHC staining of FFPE rat brain tissue with Syndecan 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Western blot testing of 1) human U-2 OS, 2) rat PC-12 and 3) mouse NIH 3T3 cell lysate with Syndecan 3 antibody. Predicted molecular weight: 46 kDa, observed molecular weight: 60-70 kDa.
IHC staining of FFPE human placental tissue with Syndecan 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain tissue with Syndecan 3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Syndecan 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Syndecan-3, also called SYND3, is a protein that in humans is encoded by the SDC3 gene. This gene was assigned to human chromosome 1p35.2. This gene can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparin sulfate chains. It may play a role in the organization of cell shape by affecting the actin cytoskeleton, and possibly by transferring signals from the cell surface in a sugar-dependent mechanism. In wildtype mice, SDC3 was expressed in hypothalamic regions that control energy balance. It was proposed that oscillation of hypothalamic SDC3 levels physiologically modulates feeding behavior.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the N-terminus of mouse Syndecan 3 (AQRWRNENFERPVDLE) was used as the immunogen for this Syndecan 3 antibody (100% rat homology).
Limitation:
This Syndecan 3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q64519