Syndecan-1 Antibody / CD138

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NSJ Bioreagents
Product Code: NSJ-R31994
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31994-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
After reconstitution, the SDC1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A431 cells with SDC1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human A549 cells with SDC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDC1 antibody.
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Western blot testing of human 1) HT1080, 2) MCF7, 3) 293, 4) HepG2 and 5) HeLa cell lysate with SDC1 antibody. Expected molecular weight: 32-95 kDa depending on glycosylation level.
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Immunofluorescent staining of FFPE human A431 cells with SDC1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with SDC1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SDC1 antibody.
Western blot testing of human 1) HT1080, 2) MCF7, 3) 293, 4) HepG2 and 5) HeLa cell lysate with SDC1 antibody. Expected molecular weight: 32-95 kDa depending on glycosylation level.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the SDC1 antibody should be determined by the researcher.
Description:
Syndecan 1 is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKE of human Syndecan-1 were used as the immunogen for the SDC1 antibody.
Limitation:
This SDC1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P18827