TAK1 Antibody / MAP3K7

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NSJ Bioreagents
Product Code: NSJ-R32443
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32443-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Application: Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the TAK1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Immunofluorescent staining of FFPE human SiHa cells with TAK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of 1) human MCF7, 2) human A431, 3) human A375, 4) rat heart, 5) mouse heart and 6) mouse NIH 3T3 cell lysate with TAK1 antibody. Predicted molecular weight: 64-69 kDa, routinely observed at 78-82 kDa.
3 / 3
Flow cytometry testing of human U-87 MG cells with TAK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAK1 antibody.
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Immunofluorescent staining of FFPE human SiHa cells with TAK1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) human MCF7, 2) human A431, 3) human A375, 4) rat heart, 5) mouse heart and 6) mouse NIH 3T3 cell lysate with TAK1 antibody. Predicted molecular weight: 64-69 kDa, routinely observed at 78-82 kDa.
Flow cytometry testing of human U-87 MG cells with TAK1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TAK1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Mitogen-activated protein kinase kinase kinase 7, also known as TAK1, is an enzyme that in humans is encoded by the MAP3K7 gene. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids RLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQ were used as the immunogen for the TAK1 antibody.
Limitation:
This TAK1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O43318