TCP1 delta Antibody / CCT4

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NSJ Bioreagents
Product Code: NSJ-R32421
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32421-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TCP1 delta antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) rat brain, 2) mouse brain and 3) human HeLa lysate with TCP1 delta antibody. Expected/observed molecular weight ~58 kDa.
2 / 10
IHC testing of FFPE human breast cancer tissue with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
3 / 10
IHC testing of FFPE mouse kidney with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
4 / 10
IHC testing of FFPE rat brain with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
5 / 10
IHC testing of frozen mouse brain tissue with TCP1 antibody.
6 / 10
IHC testing of frozen rat brain tissue with TCP1 antibody.
7 / 10
ICC testing of FFPE human SHG-44 cells with TCP1 delta antibody.
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ICC testing of FFPE mouse Neuro-2a cells with TCP1 delta antibody.
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ICC testing of FFPE rat NRK cells with TCP1 delta antibody.
10 / 10
Flow cytometry testing of human U-251 MG cells with TCP1 delta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 delta antibody.
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Western blot testing of 1) rat brain, 2) mouse brain and 3) human HeLa lysate with TCP1 delta antibody. Expected/observed molecular weight ~58 kDa.
IHC testing of FFPE human breast cancer tissue with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse kidney with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat brain with TCP1 delta antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of frozen mouse brain tissue with TCP1 antibody.
IHC testing of frozen rat brain tissue with TCP1 antibody.
ICC testing of FFPE human SHG-44 cells with TCP1 delta antibody.
ICC testing of FFPE mouse Neuro-2a cells with TCP1 delta antibody.
ICC testing of FFPE rat NRK cells with TCP1 delta antibody.
Flow cytometry testing of human U-251 MG cells with TCP1 delta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 delta antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the TCP1 delta antibody should be determined by the researcher.
Description:
T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids R452-R539 of the human protein were used as the immunogen for the TCP1 delta antibody.
Limitation:
This TCP1 delta antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P50991