TCP1 epsilon Antibody / CCT5

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NSJ Bioreagents
Product Code: NSJ-R32402
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32402-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TCP1 epsilon antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) rat testis and 2) human HeLa lysate with TCP1 epsilon antibody. Expected/observed molecular weight ~60 kDa.
2 / 7
IHC testing of FFPE human intestinal cancer tissue with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
3 / 7
IHC testing of FFPE mouse ovary with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
4 / 7
IHC testing of FFPE rat ovary with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
5 / 7
ICC testing of FFPE human LOVO cells with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
6 / 7
Flow cytometry testing of human A431 cells with TCP1 epsilon antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 epsilon antibody.
7 / 7
Immunofluorescent staining of FFPE human U-2 OS cells with TCP1 epsilon antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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Western blot testing of 1) rat testis and 2) human HeLa lysate with TCP1 epsilon antibody. Expected/observed molecular weight ~60 kDa.
IHC testing of FFPE human intestinal cancer tissue with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse ovary with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat ovary with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
ICC testing of FFPE human LOVO cells with TCP1 epsilon antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Flow cytometry testing of human A431 cells with TCP1 epsilon antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 epsilon antibody.
Immunofluorescent staining of FFPE human U-2 OS cells with TCP1 epsilon antibody (green) at 2ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the TCP1 epsilon antibody should be determined by the researcher.
Description:
CCT5 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids L443-K535 of the human protein were used as the immunogen for the TCP1 epsilon antibody.
Limitation:
This TCP1 epsilon antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P48643