TCP1 eta Antibody / CCT7

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NSJ Bioreagents
Product Code: NSJ-RQ4387
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4387-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the TCP1 eta antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) HepG2, 3) MDA-MB-231, 4) rat testis and 5) mouse testis lysate with TCP1 eta antibody at 0.5ug/ml. Predicted molecular weight: ~59 kDa.
2 / 3
Immunofluorescence staining of FFPE human U-2 OS cells with TCP1 eta antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
3 / 3
Flow cytometry testing of human HepG2 cells with TCP1 eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 eta antibody.
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Western blot testing of human 1) HeLa, 2) HepG2, 3) MDA-MB-231, 4) rat testis and 5) mouse testis lysate with TCP1 eta antibody at 0.5ug/ml. Predicted molecular weight: ~59 kDa.
Immunofluorescence staining of FFPE human U-2 OS cells with TCP1 eta antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human HepG2 cells with TCP1 eta antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TCP1 eta antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml (human recombinant protein)
Application Note:
Optimal dilution of the TCP1 eta antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
T-complex protein 1 subunit eta is a protein that in humans is encoded by the CCT7 gene. This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 6.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids Q30-D307 was used as the immunogen for the TCP1 eta antibody.
Limitation:
This TCP1 eta antibody is available for research use only.
Localization:
Cytoplasm
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q99832