TCP1 theta Antibody / CCT8

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NSJ Bioreagents
Product Code: NSJ-R32478
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32478-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Prior to reconstitution, store at 40. After reconstitution, the TCP1 theta antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) rat brain, 2) mouse brain and 3) human U20S lysate with TCP1 theta antibody. Expected/observed molecular weight ~60 kDa.
2 / 4
IHC testing of FFPE human breast cancer tissue with TCP1 theta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
3 / 4
IHC testing of FFPE mouse kidney with TCP1 theta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
4 / 4
IHC testing of FFPE rat testis with TCP1 theta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
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Western blot testing of 1) rat brain, 2) mouse brain and 3) human U20S lysate with TCP1 theta antibody. Expected/observed molecular weight ~60 kDa.
IHC testing of FFPE human breast cancer tissue with TCP1 theta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE mouse kidney with TCP1 theta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
IHC testing of FFPE rat testis with TCP1 theta antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
T-complex protein 1 subunit theta is a protein that in humans is encoded by the CCT8 gene. This gene encodes the theta subunit of the CCT chaperonin, which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of newly synthesized proteins. Alternative splicing results in multiple transcript variants of this gene. A pseudogene related to this gene is located on chromosome 1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids K421-D548 from the human protein were used as the immunogen for the TCP1 theta antibody.
Limitation:
This TCP1 theta antibody is available for research use only.
Localization:
Cytoplasmic, membranous
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P50990