TFAM Antibody / mtTFA

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NSJ Bioreagents
Product Code: NSJ-R32176
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32176-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the mtTFA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IF/ICC staining of FFPE human A431 cells with mtTFA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC testing of FFPE human lung cancer with mtTFA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 5
Western blot testing of human 1) Jurkat, 2) A431, 3) HL-60, 4) CCRF-CEM, 5) 293T and 6) SW620 lysate with mtTFA antibody at 0.5ug/ml. Expected molecular weight 24~29 kDa.
4 / 5
Western blot testing of human 1) Caco-2, 2) SW620, 3) PANC-1, 4) rat brain and 5) mouse brain lysate with mtTFA antibody. Expected molecular weight 24~29 kDa.
5 / 5
Flow cytometry testing of human Caco-2 cells with mtTFA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= mtTFA antibody.
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IF/ICC staining of FFPE human A431 cells with mtTFA antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human lung cancer with mtTFA antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Western blot testing of human 1) Jurkat, 2) A431, 3) HL-60, 4) CCRF-CEM, 5) 293T and 6) SW620 lysate with mtTFA antibody at 0.5ug/ml. Expected molecular weight 24~29 kDa.
Western blot testing of human 1) Caco-2, 2) SW620, 3) PANC-1, 4) rat brain and 5) mouse brain lysate with mtTFA antibody. Expected molecular weight 24~29 kDa.
Flow cytometry testing of human Caco-2 cells with mtTFA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= mtTFA antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the mtTFA antibody should be determined by the researcher.
Description:
TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids EMKSWEEQMIEVGRKDLLRRTIKKQRKY of human mtTFA were used as the immunogen for the mtTFA antibody.
Limitation:
This mtTFA antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q00059