Tff2 Antibody / Trefoil factor 2

NSJ Bioreagents
Product Code: NSJ-R32975
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32975-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Tff2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of mouse testis lysate with Tff2 antibody at 0.5ug/ml. Predicted molecular weight ~14 kDa.
2 / 3
IHC testing of FFPE mouse stomach tissue with Tff2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 3
IHC testing of FFPE rat stomach tissue with Tff2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Western blot testing of mouse testis lysate with Tff2 antibody at 0.5ug/ml. Predicted molecular weight ~14 kDa.
IHC testing of FFPE mouse stomach tissue with Tff2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat stomach tissue with Tff2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Direct ELISA (mouse recombinant protein): 0.1-0.5ug/ml (BSA-free format available)
Application Note:
Optimal dilution of the Tff2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids E24-Y129 was used as the immunogen for the Tff2 antibody.
Limitation:
This Tff2 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q03404