Thrombospondin 2 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30487
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30487-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Thrombospondin 2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa, 2) 22RV1, 3) U-87 MG, 4) ThP-1, 5) PC-3 and 6) Caco-2 cell lysate with Thrombospondin 2 antibody. Expected molecular weight: 130-170 kDa depending on glycosylation level.
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Western blot testing of 1) rat brain and 2) mouse brain lysate with Thrombospondin 2 antibody. Expected molecular weight: 130-170 kDa depending on glycosylation level.
3 / 4
IHC-P: Thrombospondin 2 antibody testing of human placenta tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 4
Flow cytometry testing of human U-87 MG cells with Thrombospondin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Thrombospondin 2 antibody.
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Western blot testing of human 1) HeLa, 2) 22RV1, 3) U-87 MG, 4) ThP-1, 5) PC-3 and 6) Caco-2 cell lysate with Thrombospondin 2 antibody. Expected molecular weight: 130-170 kDa depending on glycosylation level.
Western blot testing of 1) rat brain and 2) mouse brain lysate with Thrombospondin 2 antibody. Expected molecular weight: 130-170 kDa depending on glycosylation level.
IHC-P: Thrombospondin 2 antibody testing of human placenta tissue. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human U-87 MG cells with Thrombospondin 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Thrombospondin 2 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Thrombospondin 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Thrombospondin-2(THBS2) is a protein that in humans is encoded by the THBS2 gene. The protein encoded by this gene belongs to the thrombospondin family. The THBS2 is mapped to 6q27 and it is located on chromosome 17. The gene was transcribed in fibroblasts, smooth muscle cells, and an osteosarcoma cell line. It functions as a protein inhibitor of tumor growth and angiogenesis and modulates the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human Thrombospondin 2 (EAAKTEKQVCEPENPCKDK) was used as the immunogen for this Thrombospondin 2 antibody.
Limitation:
This Thrombospondin 2 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
P35442