TLR1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4250
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4250-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TLR1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Flow cytometry testing of human ThP-1 cells with TLR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=TLR1 antibody.
2 / 6
Flow cytometry testing of human K562 cells with TLR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=TLR1 antibody.
3 / 6
Western blot testing of 1) rat spleen and 2) mouse intestine lysate with TLR1 antibody at 0.5ug/ml. Predicted molecular weight ~90 kDa.
4 / 6
IHC testing of FFPE mouse spleen tissue with TLR1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 6
IHC testing of FFPE rat spleen tissue with TLR1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 6
IHC testing of FFPE human lung cancer tissue with TLR1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Flow cytometry testing of human ThP-1 cells with TLR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=TLR1 antibody.
Flow cytometry testing of human K562 cells with TLR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue=TLR1 antibody.
Western blot testing of 1) rat spleen and 2) mouse intestine lysate with TLR1 antibody at 0.5ug/ml. Predicted molecular weight ~90 kDa.
IHC testing of FFPE mouse spleen tissue with TLR1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat spleen tissue with TLR1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human lung cancer tissue with TLR1 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the TLR1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Toll-like receptor 1 (TLR1), also called TIL or CD281 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. This gene is mapped to 4p14 by fluorescence in situ hybridization. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids F350-D404 was used as the immunogen for the TLR1 antibody.
Limitation:
This TLR1 antibody is available for research use only.
Localization:
Cell membrane, cytoplasm
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15399