TLR5 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31826
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31826-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TLR5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Jurkat cell lysate with TLR5 antibody. Expected/observed molecular weight ~98 kDa.
2 / 3
IHC testing of FFPE human appendicitis tissue with TLR5 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 3
Flow cytometry testing of human PBMC with TLR5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLR5 antibody.
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Western blot testing of Jurkat cell lysate with TLR5 antibody. Expected/observed molecular weight ~98 kDa.
IHC testing of FFPE human appendicitis tissue with TLR5 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Flow cytometry testing of human PBMC with TLR5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLR5 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the TLR5 antibody should be determined by the researcher.
Description:
TLR5 encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids MKHQSIRGFVQKQQYLRWPEDFQDVGWF of human TLR5 were used as the immunogen for the TLR5 antibody.
Limitation:
This TLR5 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
O60602